Change authentication mode to 'None ' in web.config.Go to your web.config file and change following line< authentication mode="None" /> This should solve this problem.Note: If above solution does not work then make sure that the directory from where you website is running is configured as an application in IIS. To do this, you need to go into IIS and find your project folder on the server, right click to Properties. Under Application Settings click Create.Cause By default ASP.Net creates Windows authentication mode in Web.Config file. When you run this site locally on your computer, it runs fine. This is because it runs under current windows account credentials (i.e. Your windows login which may have administrative rights). But when you copy it to web server it runs under [MACHINENAME]\ASPNet account. If it does not have appropriate rights, server will throw this error[/BLUE]
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